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. 2017 Aug 9;7:7683. doi: 10.1038/s41598-017-08067-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hinge-initiated primer-based replication with one hinge-primer and PCR-like primer. The hinge-primer anneals to the target region and will be extended to double strand DNA by Bst DNA polymerase (1). The refolding to the thermodynamically more stable hairpin structure liberates the initial priming site (2–3). These steps are repeated and the sense DNA strand will be released. The PCR-like primer can anneal to the priming site and will be extended (4).