Table 2.
Variation of magnesium concentration in the hinge-amplification reaction in combination with three different primers.
| Primer combination | Template | Magnesium concentration | |||
|---|---|---|---|---|---|
| 4 mM | 6 mM | 8 mM | 10 mM | ||
| LZ-HF and LZ-HR | + | 30 ± 1 | 17 ± 1 | 25 ± 1 | 29 ± 0 |
| − | NA | 46 ± 1 | 58 ± 1 | 46 ± 1 | |
| LZ-HF and LZR-653 | + | 56 ± 2 | 34 ± 1 | 38 ± 1 | 45 ± 2 |
| − | NA | NA | NA | NA | |
| LZL-389 and LZ-HR | + | 51 ± 1 | 39 ± 2 | 46 ± 1 | 46 ± 1 |
| − | NA | NA | NA | NA | |
The test included both hinge-primer and the forward and reverse primer separately. The magnesium concentration ranges from 4 mM to 10 mM. Time-to-positivity (Tp / minutes) values are listed including standard deviation. NA represents no amplification detected.