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. 2017 Aug 9;8:212. doi: 10.1038/s41467-017-00285-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Isothermal calorimetric titration of E9 endonuclease by an Im2 mutant. Shown is a representative experiment in which wild-type E9 endonuclease was titrated with the Asn34→Ala mutant of the Im2 immunity protein. The titration was carried out in 250 mM ethylenediammonium diacetate buffer (pH 7.0) at 25 °C as described under “Methods” section