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. 2017 Aug 9;7:7703. doi: 10.1038/s41598-017-08140-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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shRNA-IGF-1R disrupts the polarized location of migrating neurons. (a) Co-electroporation of shIGF-1R/DsRed and GalT2-YFP into brains at E15 (left) decreases the proportion of DsRed-positive cells in the VZ and/or IZ with a Golgi apparatus oriented towards the radial axe fate at E17 compared to a control vector (left). Lower magnification pictures (bottom) and higher magnification insets (top) are shown. Calibration bar = 50 μm (bottom) or 25 μm (top). (b) Quantification of the experiment shown in A. Student’s t test; **p-value = 0.01. Note that in the brains suppressed for IGF-1R the number of cells facing the cortical plate is close to 60% (random distribution = 50%). n = 3 independent experiments. An average of 300 cells was scored for each condition. (c) For quantifications, we considered that a cell has the Golgi not oriented to the CP when the majority of the GalT2 staining was concentrated below the axis.