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. 2017 Aug 9;7:7709. doi: 10.1038/s41598-017-06953-y

Figure 3.

Figure 3

TDP-43 overexpressed increases stabilities of mt-tRNAAsn, mt-tRNAGln, and mt-tRNALeu(UUR). DAP-TDP-43–inducible T-REx 293 cells were treated with or without doxycycline (Dox) for 24 h and then treated with EtBr for the indicated periods. Cells were collected at 0, 6, 10, and 24 h after addition of EtBr for analysis of mt-tRNAAs n by northern blotting (a) and at 0, 10, 24, 36 h for analysis of mt-tRNAGln or mt-tRNALeu(UUR) (b). mt-12S rRNA was detected by SYBR Gold staining and MS-based analysis (b). The graphs present data for the average ± SD (three independent experiments) of staining intensities at each time point relative to 0 h. Each band intensity was normalised to that of 5S rRNA detected using SYBR Gold staining.