Figure 5.
TDP-43 overexpression causes accumulation of intermediates of mitochondrial transcript. (a) Schematic diagram of H-strand mitochondrial DNA from mt-tRNAPhe (F) to ATP6, and the corresponding mt-transcripts are shown. Nine probes used for northern blotting are indicated above the diagram. (b) Total RNA extracted from DAP-TDP-43-expressing T-REx 293 cells harvested at 0, 24, or 48 h after doxycycline (Dox) induction were analysed by northern blotting with the probes indicated under the figures. EtBr served as a negative control for the mt-transcripts. The processing intermediates of mt-transcripts identified by northern blotting are indicated under the diagram and to the right of each panel. (c), Graphical representation of band intensities for the observed mt-transcripts shown in a relative to cells without Dox induction (0 h). Data are the average ± SEM of at least three independent experiments. **P < 0.01; *P < 0.05 (paired t test).