Table 2.
Phosphoproteins in porcine LV biopsies identified with a difference in FDR-based statistical analysis.
| increased/higher phosphorylation | protein name | phosphorylation site | protein ID | -fold higher phosphorylation | p-value |
|---|---|---|---|---|---|
| at baseline with RIPC | |||||
| — | — | — | — | — | |
| at baseline with sham | |||||
| — | — | — | — | ||
| at early reperfusion with RIPC | |||||
| microtubule-associated protein 1B | 1324 | P15205 | 4.28 | 0.0000 | |
| mitogen-activated protein kinase 3 | 134 | P27361 | 3.30 | 0.0001 | |
| myosin-7 | 415; 1222; 1221 | P12883 | 6.19 | 0.0003 | |
| at early reperfusion with sham | |||||
| — | — | — | — | — | |
| with RIPC at baseline | |||||
| synaptopodin 2-like protein | 893 | Q9H988 | 3.94 | 0.0014 | |
| with RIPC at early reperfusion | |||||
| actin-binding LIM protein 1 | 286 | O14639 | 4.80 | 0.0001 | |
| adenylyl cyclase-associated protein 1 | 202 | P40123 | 7.65 | 0.0017 | |
| ADP/ATP translocase 1 | 42 | P12235 | 6.36 | 0.0002 | |
| α-crystallin B chain | 59 | P02511 | 6.45 | 0.0006 | |
| α-crystallin B chain | 19 | P02512 | 6.38 | 0.0030 | |
| ankyrin-2 | 3732 | Q01484 | 6.52 | 0.0018 | |
| ankyrin-2 | 3848 | Q01484 | 5.72 | 0.0002 | |
| BAG family molecular chaperone regulator 3 | 387 | O95817 | 3.94 | 0.0020 | |
| Bcl-2-like protein 13 | 344 | Q9BXK5 | 8.34 | 0.0005 | |
| β-taxilin | 554 | Q8N3L3 | 5.93 | 0.0000 | |
| F-actin capping protein beta subunit variant II | 263;263 | P47756 | 4.41 | 0.0020 | |
| galectin 3 | 25 | P17931 | 7.93 | 0.0000 | |
| glycogen [starch] synthase | 727 | P13807 | 8.12 | 0.0015 | |
| host cell factor 1 | 666 | P51610 | 6.89 | 0.0001 | |
| kinesin-like protein | 930 | P33176 | 8.76 | 0.0002 | |
| LIM and calponin homology domains-containing protein 1 | 718 | Q9UPQ0 | 3.53 | 0.0006 | |
| LIM domain-binding protein 3 | 189; 190; 267 | O75112 | 17.65 | 0.0009 | |
| mitogen-activated protein kinase 1 | 90; 152 | P28482 | 4.17 | 0.0003 | |
| mitogen-activated protein kinase 3 | 134 | P27361 | 4.60 | 0.0005 | |
| myosin-7 | 210; 629 | P12883 | 7.19 | 0.0023 | |
| PGC-1 and ERR-induced regulator in muscle protein 1 | 138 | Q5SV97 | 5.17 | 0.0008 | |
| phosphatidylethanolamine-binding protein 1 | 185 | P30086 | 9.38 | 0.0011 | |
| proteasome inhibitor PI31 subunit | 152 | Q92530 | 5.59 | 0.0012 | |
| protein phosphatase 1G | 526 | O15355 | 4.75 | 0.0011 | |
| rho guanine nucleotide exchange factor 2 | 619 | Q92974 | 6.37 | 0.0031 | |
| selenocysteine lyase | 17 | Q96I15 | 8.13 | 0.0001 | |
| synaptopodin 2-like protein | 722 | Q9H987 | 3.30 | 0.0008 | |
| synaptopodin-2 | 250 | Q9UMS6 | 9.41 | 0.0001 | |
| synemin | 1250 | O15061 | 7.67 | 0.0011 | |
| synemin | 1020 | O15062 | 6.75 | 0.0002 | |
| trafficking kinesin-binding protein 1 | 377 | Q9UPV9 | 6.28 | 0.0031 | |
| uncharacterized protein | 58 | F1RZW0 | 6.40 | 0.0005 | |
| uncharacterized protein | 66 | F1RZW0 | 6.80 | 0.0000 | |
| uncharacterized protein | 45 | F1SC49 | 6.32 | 0.0010 | |
| uncharacterized protein | 477 | F1SE25 | 4.71 | 0.0024 | |
| uncharacterized protein | 46; 230 | F1SMV6 | 5.58 | 0.0002 | |
| uncharacterized protein | 223 | I3L8Q0 | 7.94 | 0.0001 | |
| uncharacterized protein | 31 | I3LAR5 | 8.06 | 0.0005 | |
| uncharacterized protein | 297 | I3LAR5 | 6.88 | 0.0000 | |
| uncharacterized protein | 422 | I3LAR5 | 5.77 | 0.0009 | |
| uncharacterized protein | 384 | I3LI59 | 6.63 | 0.0001 | |
| uncharacterized protein | 1205 | I3LKE2 | 3.94 | 0.0009 | |
| uncharacterized protein | 443; 283 | I3LPY1 | 9.99 | 0.0001 | |
| uncharacterized protein | 432; 272 | I3LRX7 | 9.39 | 0.0006 | |
| uncharacterized protein | 1343 | I3LUY9 | 6.84 | 0.0013 | |
| uncharacterized protein | 234; 593 | I3LUY9 | 7.77 | 0.0025 | |
| UV excision repair protein RAD23 homolog B | 160 | P54727 | 7.57 | 0.0005 | |
| with sham at baseline | |||||
| actin-binding LIM protein 1 | 471 | O14639 | 3.71 | 0.0036 | |
| afadin | 1274 | P55196 | 4.92 | 0.0021 | |
| α-1-syntrophin | 201 | Q13424 | 6.94 | 0.0000 | |
| α-1-syntrophin | 189 | Q13425 | 7.12 | 0.0002 | |
| α-endosulfine | 101; 109 | O43768 | 5.29 | 0.0044 | |
| α-endosulfine | 59; 67; 62 | O43768 | 5.29 | 0.0014 | |
| AP-4 complex accessory subunit Tepsin | 402 | Q96N21 | 2.29 | 0.0007 | |
| Arf-GAP domain and FG repeat-containing protein 1 | 95 | P52594 | 5.84 | 0.0012 | |
| ATP-binding cassette sub-family F member 1 | 228 | Q8NE71 | 2.74 | 0.0014 | |
| Bcl-2-associated transcription factor 1 | 397 | Q9NYF8 | 3.75 | 0.0055 | |
| β-2-syntrophin | 95 | Q13425 | 4.57 | 0.0011 | |
| calnexin | 565 | P27824 | 4.41 | 0.0006 | |
| calumenin | 47 | O43852 | 8.94 | 0.0006 | |
| cAMP-dependent protein kinase type I-alpha regulatory subunit | 82 | P10644 | 3.45 | 0.0007 | |
| C-type lectin domain family 14 member A | 493 | Q86T13 | 4.09 | 0.0056 | |
| E3 ubiquitin-protein ligase MGRN1 | 516 | O60291 | 2.53 | 0.0015 | |
| formin-like protein 3 | 774; 745; 584 | Q8IVF7 | 6.57 | 0.0022 | |
| heat shock protein beta-8 | 24 | Q9UJY1 | 8.52 | 0.0001 | |
| MICOS complex subunit MIC60 | 88 | Q16891 | 5.77 | 0.0008 | |
| microtubule-associated protein 1B | 1324 | P46821 | 4.05 | 0.0047 | |
| mitochondrial import inner membrane translocase subunit TIM44 | 134 | O43615 | 7.66 | 0.0000 | |
| myopalladin | 928 | Q86TC9 | 4.17 | 0.0010 | |
| myosin-7 | 415; 1222; 1221 | P12883 | 5.93 | 0.0003 | |
| nexilin | 16; 80 | Q0ZGT2 | 3.37 | 0.0032 | |
| nexilin | 300; 364 | Q0ZGT2 | 6.35 | 0.0013 | |
| nuclear receptor-binding protein | 433 | Q9UHY1 | 5.36 | 0.0067 | |
| protein LBH | 64 | Q53QV2 | 4.49 | 0.0047 | |
| protocadherin-7 | 975 | O60245 | 5.20 | 0.0018 | |
| R3H domain-containing protein 2 | 425 | Q9Y2K5 | 4.35 | 0.0057 | |
| RNA-binding protein 20 | 686; 690 | Q5T481 | 4.61 | 0.0062 | |
| serine/threonine-protein kinase SIK3 | 461 | Q9Y2K2 | 4.25 | 0.0039 | |
| solute carrier family 16. member 1 (Monocarboxylic acid transporter 1) tv1 | 484 | P53985 | 3.69 | 0.0002 | |
| SUN domain-containing protein 2 | 55 | Q9UH99 | 5.21 | 0.0034 | |
| supervillin | 305; 264 | O95425 | 7.98 | 0.0097 | |
| thioredoxin-related transmembrane protein 1 | 245 | Q9H3N1 | 3.56 | 0.0044 | |
| uncharacterized protein | 37 | F1RVC9 | 2.50 | 0.0008 | |
| uncharacterized protein | 179; 182 | F1S2M0 | 7.95 | 0.0019 | |
| uncharacterized protein | 5 | F1S5R6 | 4.67 | 0.0008 | |
| uncharacterized protein | 1368 | I3L8Q0 | 3.19 | 0.0039 | |
| uncharacterized protein | 887; 1219; 1221 | I3LA95 | 5.90 | 0.0056 | |
| uncharacterized protein | 254 | I3LBD0 | 3.41 | 0.0002 | |
| zinc finger Ran-binding domain-containing protein 2 | 153 | O95218 | 4.13 | 0.0048 | |
| 14 kDa phosphohistidine phosphatase | 36 | Q9NRX4 | 8.38 | 0.0074 | |
| with RIPC at early reperfusion | |||||
| adenylyl cyclase-associated protein | 202 | P40123 | 4.95 | 0.0012 | |
| ADP/ATP translocase 1 | 42 | P12235 | 7.23 | 0.0001 | |
| ankyrin repeat and MYND domain-containing protein 2 | 403; 439 | Q8IV38 | 5.79 | 0.0002 | |
| ankyrin-1 | 12 | P16157 | 6.98 | 0.0019 | |
| band 4.1-like protein 1 | 667 | Q9H4G0 | 4.63 | 0.0017 | |
| Bcl-2-like protein 13 | 344 | Q9BXK5 | 8.83 | 0.0031 | |
| β-taxilin | 554 | Q8N3L3 | 3.67 | 0.0001 | |
| calpastatin | 312; 244; 222 | P20810 | 5.08 | 0.0014 | |
| caprin-1 | 306 | Q14444 | 5.53 | 0.0017 | |
| clustered mitochondria protein homolog | 1253 | O75153 | 5.00 | 0.0072 | |
| coiled-coil domain-containing protein 124 | 141 | Q96CT7 | 4.36 | 0.0054 | |
| desmin | 45 | P01019 | 6.72 | 0.0013 | |
| DNA-(apurinic or apyrimidinic site) lyase | 136 | P27695 | 6.03 | 0.0063 | |
| E3 ubiquitin-protein ligase HUWE1 | 3756; 3758; 83 | Q7Z6Z7 | 6.30 | 0.0001 | |
| F-actin capping protein beta subunit variant II | 263 | P47756 | 3.64 | 0.0039 | |
| fragile X mental retardation syndrome-related protein 2 | 654 | P51116 | 2.83 | 0.0005 | |
| galectin | 25 | Q3ZCW2 | 4.69 | 0.0000 | |
| glutamate 5-kinase 1 | 581 | Q98EZ4 | 9.18 | 0.0098 | |
| glycogen [starch] synthase | 727 | P13807 | 4.58 | 0.0003 | |
| heat shock 70 kDa protein 4 | 76 | O88600 | 10.88 | 0.0046 | |
| heat shock protein beta-1 | 178 | P04792 | 5.38 | 0.0002 | |
| heterogeneous nuclear ribonucleoproteins A2/B1 | 29 | P22626 | 8.01 | 0.0008 | |
| kinesin-like protein | 930 | P33176 | 4.68 | 0.0058 | |
| LIM domain-binding protein 3 | 190 | O75115 | 8.60 | 0.0009 | |
| LIM domain-binding protein 3 | 267 | O75116 | 7.28 | 0.0014 | |
| LIM domain-binding protein 3 | 116 | O75117 | 5.00 | 0.0039 | |
| MAPK-interacting and spindle-stabilizing protein-like | 15 | Q8NDC0 | 4.95 | 0.0015 | |
| melanoma-associated antigen D2 | 82 | Q9UNF1 | 5.25 | 0.0054 | |
| mitochondrial antiviral-signaling protein | 220 | Q7Z434 | 6.11 | 0.0000 | |
| mitochondrial antiviral-signaling protein | 202 | Q7Z434 | 6.08 | 0.0032 | |
| myeloid leukemia factor 1 | 33 | P58340 | 9.82 | 0.0016 | |
| myocardial zonula adherens protein | 39 | P0CAP1 | 5.22 | 0.0068 | |
| myotilin | 145 | Q9UBF9 | 6.82 | 0.0004 | |
| myotilin | 496 | Q9UBF9 | 5.25 | 0.0074 | |
| nexilin | 248; 312 | Q0ZGT2 | 9.77 | 0.0003 | |
| nucleosome assembly protein 1-like 4 | 5 | Q99733 | 5.81 | 0.0026 | |
| PGC-1 and ERR-induced regulator in muscle protein 1 | 138 | Q149B8 | 5.64 | 0.0041 | |
| phosphatidylethanolamine-binding protein 1 | 185 | P31044 | 7.54 | 0.0012 | |
| phospholipase A-2-activating protein | 324 | Q9Y263 | 6.76 | 0.0026 | |
| pinin | 66 | Q9H307 | 4.54 | 0.0019 | |
| progesterone receptor membrane component 2 | 104; 46 | O15173 | 10.87 | 0.0004 | |
| proteasome inhibitor PI31 subunit | 152 | Q92530 | 7.05 | 0.0091 | |
| protein NDRG2 | 330 | Q9UN36 | 6.59 | 0.0082 | |
| protein phosphatase 1 regulatory subunit 7 | 24 | Q15435 | 5.33 | 0.0029 | |
| rho guanine nucleotide exchange factor 2 | 619 | Q92974 | 4.14 | 0.0006 | |
| selenocysteine lyase | 17 | Q96I15 | 6.71 | 0.0003 | |
| sequestosome-1 | 272 | Q13501 | 3.97 | 0.0028 | |
| sodium/potassium-transporting ATPase subunit alpha | 15; 16 | P05023 | 6.62 | 0.0021 | |
| starch-binding domain-containing protein 1 | 173 | O95210 | 7.92 | 0.0005 | |
| striatin-3 | 276 | Q13033 | 3.59 | 0.0026 | |
| supervillin | 1091; 961 | O95425 | 5.14 | 0.0054 | |
| synaptopodin 2-like protein | 939 | Q9H987 | 3.27 | 0.0011 | |
| synemin | 738 | O15063 | 7.83 | 0.0008 | |
| synemin | 580 | O15064 | 6.23 | 0.0007 | |
| synemin | 1250 | O15065 | 6.08 | 0.0015 | |
| synemin | 873 | O15066 | 3.73 | 0.0029 | |
| transforming acidic coiled-coil-containing protein 2 | 1594 | O95359 | 4.72 | 0.0069 | |
| transforming acidic coiled-coil-containing protein 2 | 2420; 2375; 602 | O95359 | 3.42 | 0.0004 | |
| transgelin | 172 | P37802 | 4.56 | 0.0021 | |
| uncharacterized protein | 597; 915; 828 | F1RZW0 | 4.05 | 0.0015 | |
| uncharacterized protein | 91 | F1SMV6 | 7.23 | 0.0037 | |
| uncharacterized protein | 21 | I3L5C0 | 5.93 | 0.0004 | |
| uncharacterized protein | 223;223 | I3L8Q0 | 8.23 | 0.0011 | |
| uncharacterized protein | 31 | I3L8Q0 | 7.72 | 0.0008 | |
| uncharacterized protein | 63 | I3L9T1 | 5.50 | 0.0022 | |
| uncharacterized protein | 297 | I3L9T1 | 5.08 | 0.0001 | |
| uncharacterized protein | 934 | I3L9T1 | 6.52 | 0.0065 | |
| uncharacterized protein | 384 | I3LAR5 | 4.80 | 0.0011 | |
| uncharacterized protein | 1205 | I3LAR5 | 4.25 | 0.0063 | |
| uncharacterized protein | 443; 283 | I3LAR5 | 7.15 | 0.0017 | |
| uncharacterized protein | 1738 | I3LBD6 | 5.92 | 0.0009 | |
| uncharacterized protein | 355 | I3LGU7 | 4.40 | 0.0026 | |
| uncharacterized protein | 265 | I3LGU7 | 8.57 | 0.0009 | |
| uncharacterized protein | 2451 | I3LKE2 | 4.81 | 0.0009 | |
| uncharacterized protein | 2227 | I3LU02 | 4.71 | 0.0006 | |
| uncharacterized protein | 234; 593 | I3LUY9 | 5.72 | 0.0015 | |
| uncharacterized protein | 186 | I3LUY9 | 3.96 | 0.0013 | |
| uncharacterized protein | 182 | I3LUY9 | 3.71 | 0.0018 | |
| UV excision repair protein RAD23 homolog B | 160 | P54727 | 5.80 | 0.0042 | |
| xin actin-binding repeat-containing protein 1 | 205 | Q702N8 | 3.52 | 0.0028 | |
| Y-box-binding protein 3 | 45 | P16989 | 7.29 | 0.0044 |
All phosphoproteins with a false discovery rate (FDR)-based statistical difference between remote ischemic preconditioning (RIPC) and sham at baseline or at early reperfusion, respectively, or difference between baseline and early reperfusion with RIPC or with sham after phosphopeptide enrichment of porcine left ventricular biopsies. All proteins were compared by unpaired (between RIPC and sham) Student’s t-tests.