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. 2017 Aug 9;7:7650. doi: 10.1038/s41598-017-07933-y

Figure 2.

Figure 2

Basal ATPase activity and basal Pi-release of WT and mutant myosins. (a) The basal ATPase rate was determined in the absence of actin. Error bars give the standard deviation for 3–6 independent experiments. The differences obtained for K265E and K265Q were highly significant (** for p < 0.005). (b) The basal Pi-release rate k -4 is the rate limiting step of the basal Dd myosin-2 ATPase cycle and was measured using a fluorescent Pi detection system. The obtained kinetic parameters are summarized in Table 1(a) and Table 2(b).