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. 2017 Aug 9;7:7650. doi: 10.1038/s41598-017-07933-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Actin activation of P_i-release for WT myosin and K265E mutant. (a) Averaged fluorescence traces for P_i-release at 25 µM F-actin are shown for the WT and K265E. (b) The P_i-release was determined in the presence of F-actin in a range of 0 to 25 µM. Error bars report the standard deviation and rate constants are listed in Table 2.