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. 2017 Aug 9;7:7650. doi: 10.1038/s41598-017-07933-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ADP release from myosin. (a) Averaged fluorescence traces of mant-ADP release after addition of excess (1 mM) ATP to a complex of 1 µM myosin and 20 µM mant-ADP (for clarity, only WT and K265Q data are shown). (b) The bar graph shows the ADP release rates and statistical significance for all four constructs. Error bars report the standard deviation and rate constants are listed in Table 2.