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. 2017 Aug 9;7:7671. doi: 10.1038/s41598-017-08110-x

Figure 1.

Figure 1

PrPC coupling to the NADPH oxidase-TACE signaling pathway promotes TNFR1 shedding. (a) Time-course accumulation of sTNFR1 in the cell culture medium of 1C11 cells and 1C115-HT neuronal cells upon PrPC ligation with SAF61 PrP antibody (10 μg ml−1). TNFR1 shedding induced by PrP antibodies is abolished upon inhibition of NADPH oxidase with DPI (100 μM) or TACE with TAPI-2 (100 μM). *p < 0.01 vs. nontreated cells. **p < 0.01 vs. cells exposed to SAF61 antibody. (b–d) Immunofluorescence experiments and quantification histograms showing progressive TNFR1 depletion at the cell surface of 1C11 cells (b), 1C115-HT cells (c), and primary CGNs (d) exposed to SAF61 PrP antibody and cancellation upon addition of TAPI-2. Scale bar = 50 μm. # p < 0.05 vs. nontreated cells. *p < 0.05 vs. cells exposed to SAF61 antibody alone. Data shown are the mean ± SEM from three experiments performed in triplicate.