Table 1.
The settings of filters for fluorescence imaging
| Excitation filter (nm) | Dichroic mirror (long pass; nm) | Emission filter (nm) | |
|---|---|---|---|
| CFP YFP (FRET) |
420/20 | 450 | 475/40 535/25 |
| GFP | 495/10 | 515 | 535/25 |
| mCherry | 560/40 | 595 | 653/95 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.