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. 2017 Aug 9;7:7654. doi: 10.1038/s41598-017-06837-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-lapse phase imaging of HeLa cell division over a long period (60 h). (a) Representative quantitative phase images at different time points. (b) The change of cell number and confluence ratio over the culture passage period. (c) and (d) show the magnified views corresponding to two regions of interest (Area 2 and Area 3). (e) 10 selected time-lapse phase images and the corresponding 3D renderings showing the morphological features of a dividing cell (Area 1) at different stages of mitosis.