Table I.

Characterization of 12 primer pairs for amplifying polymorphic microsatellite loci in crested macaques with PCR conditions, deviation from Hardy–Weinberg equilibrium, and estimated null allele frequency

Locus	Repeat pattern	Length of PCR product Dublin Zoo (bp)	Length of PCR product Tangkoko (bp)	Annealing temperature (°C)	Hardy–Weinberg deviation	Estimated null allele frequency	Primer sequence (5′–3′) (including modified primers)	Reference
D1S548	Tetra	181–201	185–209	58	n.s.	−0.0394	F: GAACTCATTGGCAAAAGGAA R: GCCTCTTTGTTGCAGTGATT	Lathuillière et al. 2001
D3S1768	Tetra	129–137	129–157	58	n.s.	−0.046	F: GGTTGCTGCCAAAGATTAGA R: AACTACATGATTCTAGCACA	Lathuillière et al. 2001
D5S1457	Tetra	123, 127, 131	123–139	60	n.s.	−0.0609	F: TAGGTTCTGGGCATGTCTG R: TTGCTTGGCACACTTCAGG	Bayes et al. 2000
D6S493*	Tetra	261–269b	139–159c	58	n.s.	−0.0374	F: GCAACAGTTTATGCTAAAGC R: TTCCATGGCAGAAATTGTTT	Nürnberg et al. 1998
D6S501*	Tetra	163–179b	129–145c	58	n.s.	−0.0345	F: GCTGGAAACTGATAAGGGCT R: CTTTATCTTTAATATAGGATTATTGG	Lathuillière et al. 2001
D7S2204	Tetra	171–247	220–268	58	n.s.	−0.0579	F: TCATGACAAAACAGAAATTAAGTG R: AGTAAATGGAATTGCTTGTTACC	Lathuillière et al. 2001
D10S1432	Tetra	137–145	132–148	58	n.s.	−0.0773	F: CAGTGGACACTAAACACAATCC R: TAGATTATCTAAATGGTGGATTTCC	Lathuillière et al. 2001
D11S925	Di	205–221	179–237	60	n.s.	−0.0379	F: GAACCAAGGTCGTAAGTCC R: TAGACCATTATGGGGGCAAA	Lathuillière et al. 2001
D12S67a	Tetra	135,177–193b	159–185c	58	n.s.	−0.0262	F: GCAACAGTTTATGCTAAAGC R: TGTTGTTCAAGGGTCAAATG	Nürnberg et al. 1998
D13S765a	Tetra	220, 224, 232b	137–165c	58	n.s.	−0.0512	F: TGTAACTTACTTCAAATGGCTCA R: ATTTACCTAACATTTCACCCATC	Zhang et al. 2001
D14S255a	Di	173–185b	91–113c	60	n.s.	−0.0142	F: AGCTTCCAATACCTCACCAA R: CTCTTAGTGGTCATTCTCAC	Nürnberg et al. 1998
D18S536	Tetra	144–152	144–164	58	n.s.	−0.0491	F: ATTATCACTGGTGTTAGTCCT R: CACAGTTGTGTGAGCCAGT	Kümmerli and Martin 2005

F indicates forward primers and R indicates reverse primers. n.s. = no significant deviation.

^aPrimers of this marker were modified to be specific to crested macaques.

^bBefore primer modification.

^cAfter primer modification.