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. 2017 Aug 8;8:1352. doi: 10.3389/fpls.2017.01352

FIGURE 3.

FIGURE 3

MKKK20 expression pattern monitored by the GUS reporter gene. GUS reporter gene expression under the control of the MKKK20 promoter in flower buds and seedlings. (A–D) Flower developmental stages. (A) Stage 11. (B) Stage 12. (C) Stage 13. (D) Stage 14 (according to Smyth et al., 1990). Strong staining is observed in pollen of maturing flowers. Expression tends to become stronger between stage 11 (A) and at anthesis (D). Global visualization of flower development, after manual opening, confirmed that pollen grains (P) exhibit strong MKKK20 expression, in addition to carpel (C), style (S), nectaries (N), petals (Pe), and sepals (Se), the two later with lesser intensities as they mature (A–D). (D) While pollen expression last after pollination in pollen tube (PT), staining decreases in the others flower organs. Expression can be detected also in some epidermal cells and vascular tissues. (E–G) Expression in cotyledons. (H) Expression in hypocotyl and roots. (I) Expression in companion cells of phloem tissues of roots. Scale bar: 200 μm, except for (H) (5 mm) and (I) (100 μm).