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. 2017 Aug 8;8:1352. doi: 10.3389/fpls.2017.01352

FIGURE 4.

FIGURE 4

Kinase subcellular localization. Characterization of the MKKK20, MKK3, and MPK18 nuclear and cytosolic localization. The coding regions of the three kinases were fused in-frame to the N-terminus of the GFP reporter in the pMDC83 vector (Curtis and Grossniklaus, 2003). Epifluorescence (A,C,E,G,I,K) and bright field (B,D,F,H,J,L) images of onion leaf epidermal cells show cytosolic and nuclear localization for all three kinases. Scale bar: 50 μm.