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. 2017 Aug 8;8:1373. doi: 10.3389/fpls.2017.01373

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Copyright © 2017 Chen, Zhang, Zhu, Huang, Zhu, Tang, Han, Li, Sun and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of heat stress and L-Ascorbate (Asc) on OJIP fluorescence transients in tall fescue leaves. After the reagent treatments, the petiole of the leaves was immersed 1 cm deep within distilled water kept in a falcon tube with Asc (24Asc and 44Asc) or not (24Ct and 44Ct). Then the tubes were placed in a growth cabinet with the temperature of 24°C (24Ct and 24Asc) or 44°C (44Ct and 44Asc), and light intensity of 100 μmol photons m^-2 s^-1, and a humidity of 80% for 4 h. Then after a 25-min dark-adaption period at 24°C, the OJIP curves were induced by a red light provided by a PAM 2500. Chl a fluorescence emissions were recorded by the instrument and the most typical curves are shown here.