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Effects of Asc on the electron donor side of PSII under heat stress. Tall fescue thylakoids were incubated with water (Ct) or 20 mM Asc (Asc) in a 44°C water bath for 1 min to inactivate the oxygen evolution complex. Then, 20 μL of thylakoids (200 μg Chl/mL) was dropped onto a nitrocellulose membrane, and after a 20-min dark adaption period, Chl a fluorescence transients were induced and recorded by two 5-ms saturated pulses that were spaced 200 ms apart.
