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. 2017 Aug 8;8:1373. doi: 10.3389/fpls.2017.01373

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Copyright © 2017 Chen, Zhang, Zhu, Huang, Zhu, Tang, Han, Li, Sun and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of heat stress on the APX activity (A) and production of ASC (B). Tall fescue leaves were vacuum infiltrated with water (24Ct, 44Ct) for 15 min in the dark. Then, they were moved into a growth cabinet with a temperature of 24 or 44°C, a light intensity of 100 μmol photons m^-2 s^-1 and a humidity of 80% for 4 h. Afterward, tall fescue leaves were ground in liquid nitrogen and used to determine the concentration of Asc as well as APX activity. Values are provided as the means ± SD (n = 5), and different letters indicate statistically significant difference at P < 0.05 among the treatments according to Duncan’s multiple range tests.