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. 2017 Aug 8;11:228. doi: 10.3389/fncel.2017.00228

Figure 2.

Figure 2

Resveratrol attenuated acute neurodegeneration after ICH. To estimate the number of Fluoro jade B and TUNEL positive cells, we used three coronal sections of thickness 20-μm (one from the collagenase injection site and other two from 0.25 mm anterior and 0.25 mm posterior to the injection site) per animal and the respective staining was performed as described in methods at 24 h post ICH. The schematic diagram (A) depicts a coronal brain section and the areas (the square black boxes) around the hematoma that were examined for Fluoro Jade B /TUNEL-positive cells. CTX, cortex; BG, basal ganglia. The positive cells were counted with the aid of image J software and the average number of cells per area is provided. The representative confocal images (scale bar, 20 μm; B,C) and the respective quantification demonstrate significantly reduced number of both Fluoro Jade B and TUNEL positive cells after ICH upon Resveratrol (10 mg/kg; i.v) treatment in comparison to vehicle treated controls (**p < 0.01; D,E). n = 3–4/group.