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. 2017 Aug 8;11:232. doi: 10.3389/fncel.2017.00232

Figure 5.

Figure 5

Deletion of Cav1.4 decreases RS1 distribution in the retina. (A) The upper panel shows images taken at a lower magnification (20×) of WT (a1-a5) and Cav1.4−/− (b1-b5) retinal sections that were double immunostained with antibodies against Cav1.4 and Ribeye. The lower panel shows images taken at a higher magnification (40×) of WT (c1-c5) and Cav1.4−/− (d1-d5) retinal sections stained for Cav1.4 and Ribeye. The scale bar represents 50 μm. (B) The upper panel shows images of WT retina stained with RS1 taken at lower (20×; a1-a3) and higher (40×; b1-b3) magnifications. Likewise, the lower panel shows images of Cav1.4−/− retinal sections stained with RS1 at lower (20×; c1-c3) and higher (40×; d1-d3) magnifications. The scale bar represents 50 μm. DAPI stains the nuclei. BF, bright field; IS, photoreceptor inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer.