| The fragmented and primed mRNA | 10 μL |
| (Pink) Murine RNase Inhibitor | 0.5 μL |
| Actinomycin D (0.1 μg/μL) | 5 μL |
| (Pink) ProtoScript II Reverse Transcriptase | 1 μL |
| Nuclease free water | 3.5 μL |
|
| |
| Final volume | 20 μL |
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