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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Methods Mol Biol. 2017;1537:17–36. doi: 10.1007/978-1-4939-6685-1_2
The size selected cDNA 20 μL
(Blue) NEBNext USER Enzyme 3 μL
(Blue) NEBNext High-Fidelity PCR Master Mix, 2× 25 μL
(Blue) Universal PCR Primer (25 uM) 1 μL
(Blue) Index (X) Primer (25 uM)* 1 μL
Total volume 50 μL