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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Methods Mol Biol. 2017;1537:17–36. doi: 10.1007/978-1-4939-6685-1_2
User Digestion 37°C 15 min 1 cycle
Initial Denaturation 98°C 30 sec 1 cycle
Denaturation 98°C 10 sec
Annealing 65°C 30 sec 15 cycle
Extension 72°C 30 sec
Final Extension 72°C 5 min 1 cycle
Hold 4°C