| Initial Denaturation | 94°C | 30 sec 1 cycle |
| Denaturation | 94°C | 15 sec |
| Annealing | 62°C | 30 sec 15 cycles |
| Extension | 70°C | 15 sec |
| Final Extension | 70°C | 5 min 1 cycle |
| Hold | 4°C |
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