| Total Saliva RNA + ERCC spike-in (4.5 μL of RNA + 0.5 μL Spike-in) | 5 μL |
| (Pink) NEBNext First Strand Synthesis Reaction Buffer (5×) | 4 μL |
| (Pink) NEBNext Random Primers | 1 μL |
|
| |
| Total volume | 10 μL |
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