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. Author manuscript; available in PMC: 2017 Aug 10.
Published in final edited form as: Nature. 2016 Apr 4;532(7600):527–530. doi: 10.1038/nature17391

Extended Data Figure 1. Assessment of σ1 functional properties and biochemical quality.

Extended Data Figure 1

a, Saturation binding curve to measure Kd for 3H (+)-pentazocine, with points shown as mean +/− SEM. b, Competition binding measurement of affinities for the two co-crystallized ligands with points shown as mean +/− SEM. c, Summary of binding affinities with 95% confidence intervals for Kd/Ki values. d, Analysis of receptor purity by SDS-PAGE. e, Analytical size exclusion of purified σ1 receptor in LMNG/CHS detergent buffer on a Superdex 200 column.