Figure 3.

Correlation of AChR distribution with specific pre- and postsynaptic markers at NMJs. A, B, Colocalization of AChRs (white) with various synaptic markers (magenta, threshold). Representative fluorescent images are shown on the right and the schematics on the right depict the known localization of each synaptic marker (magenta line) with respect to the junctional folds. Scale bars, 5 µm. The representative intensity profiles of AChRs (gray) and each of the synaptic markers (magenta) are shown in B. Four markers were examined: Rapsyn, an intracellular AChR scaffolding protein; piccolo, an active zone component; integrin α7, an integrin subunit involved in adhesion in NMJs. C, Quantification of the colocalization of each marker with AChRs. One-way ANOVA analysis: p = 2.22 × 10⁻⁵ (n = 4). Error bars represent the SD. Bonferroni analysis: *p = 0.012, ***p < 0.004.