Figure 3.

KMT2A regulated cancer stem cell marker expression and tumorsphere formation through hTERT signaling. (a and b) Human melanoma A375 cells were transfected with KMT2A shRNAs or KMT2A overexpression plasmid. After 48 h of transfection, the levels of Nanog, oct-4 and sox-2 were analyzed by western blot. β-actin served as the loading control. Also, the tumorsphere formation ability was tested, and representative images were displayed. (c) hTERT, Nanog, oct-4 and sox-2 expression was detected by western blot in A375 cells with KMT2A knockdown alone or with KMT2A knockdown and hTERT overexpression. (d) The tumorsphere formation ability in the cells in (c) was examined. Cells with representative morphology were shown. For quantification, more than 100 cells were inspected per experiment