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. 2017 Jul 6;8(7):e2912. doi: 10.1038/cddis.2017.289

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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SPK2 interacts with Bcl-2 in HT22 cells. (a) Stable transfected LV-SPK2-HT22 cell lysates were immune-precipitated with anti-HA, separated by SDS-PAGE, and subjected to western blot analysis with anti-Bcl-2. (b) Cell lysates were immunoprecipitated with anti-Bcl-2, separated by SDS-PAGE, and subjected to western blot analysis with anti-HA. (c) GST-Bcl-2 or GST bound to glutathione-agarose, incubated with lysates of LV-HA-SPK2-transfected HT22 cells and subjected to western blot analysis with anti-HA. n=3 independent experiments