Figure 6.

Suppression of renal NLRP3 inflammasome activation by IL-22 gene therapy in mice with established nephropathy. (a) Serum levels of human IL-1β in patients with DN and age-matched healthy controls measured by ELISA. Numbers of human serum samples analyzed in each group were indicated at the top of each bar. (b) Serum levels of murine IL-1β in mice with DN and age-matched normal controls measured by ELISA (N=5). (c) Relative renal cytosolic caspase-1 activity (N=5). (d) Representative immunoblots and quantitative analysis of renal cytosolic NLRP3, cleaved caspase-1, and IL-1β expression from three individual experiments. Densitometric values of immunoreactive bands were normalized to those of β-actin and the results were expressed as fold changes. (e) Representative immunoblots and quantitative analysis of NLRP3, cleaved caspase-1, and IL-1β expression in high glucose-induced renal glomerular mesangial cells from three independent experiments. Densitometric values of immunoreactive bands were normalized to those of β-actin and the results were expressed as fold changes. *P<0.05; **P<0.01