Figure 4.

Concomitant loss of BIM or PUMA fails to rescue anaemia caused by acute deletion of Bclx. (a) Lethally irradiated GFP⁺ recipient mice were reconstituted with ‘test’ bone marrow of the indicated genotypes (GFP⁻). At 8 weeks after reconstitution a mandible bleed was taken from each mouse and analysed to confirm successful haematopoietic reconstitution and to establish baseline blood cell counts. Mice were then treated with tamoxifen (TAM, red arrows). Peripheral blood and spleens were collected for analysis 1 month after TAM administration. (b) Test red blood cell contribution at 8 weeks, prior to TAM treatment. n=6–8 (N=3–4 bone marrow donors); mean±S.E.M shown. (c) Red blood cell counts and spleen cellularity after TAM treatment. n=6–14 (N=3–7 bone marrow donors); mean±S.E.M. (d) In vitro Caspase-9 activity in FACS-sorted reticulocytes, control versus A-1331852-treated (1 μM). a.u. denotes arbitrary units. Significant differences as shown, determined by unpaired Student’s t-test