Figure 3.

Effect of high glucose or Mcc950 on HREC viability and proliferation. (a) Dose–response effect of high glucose on HREC viability and proliferation. HRECs viability were tested in normal glucose condition (5.5 mM) or high glucose(15, 30, 50 mM) condition for 72 h. Data are shown as the mean±S.E.M., n=8 technical replicates. One-way ANOVA followed by Tukey’s multiple comparison test. *P<0.01 versus control. (b) Mcc950 (0.1,1,10,100 μM) treatment for up to 48 h has no significant effect on cell viability and proliferation. Data are shown as the mean±S.E.M., n=3 technical replicates. One-way ANOVA followed by Tukey’s multiple comparison test. (c) Effect of Mcc950 on HRECs treated with 30 mM high glucose for 72 h. Cell survival shows significantly rescued by Mcc950 (0.1,1,10,100 μM) in high-glucose condition. Data are shown as the mean±S.E.M., n=8 technical replicates. One-way ANOVA followed by Tukey’s multiple comparison test. *P<0.01 versus control, ^#P<0.01 versus 30 mM high-glucose group