Figure 4.

Mcc950 pretreatment attenuated high-glucose-mediated IL-1β secretion. (a) Measurement of IL-1β released from HRECs incubated in a media that contained 5.5, 30 mM glucose for 24,48 or 72 h, respectively. Data are shown as the mean±S.E.M., n=3 technical replicates. One-way ANOVA followed by Tukey’s multiple comparison test. *P<0.01 versus control, ^#P<0.01 versus 24 h high-glucose treatment. (b) Concentration-dependent effect of Mcc950 on HRECs in 30 mM glucose condition for 72 h. Data are shown as the mean±S.E.M., n=3 technical replicates. One-way ANOVA followed by Tukey’s multiple comparison test. *P<0.05, ^#P<0.01 versus control