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. 2017 Jul 20;8(7):e2941. doi: 10.1038/cddis.2017.308

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Mcc950 inhibited the activation of NLRP3 inflammasome by western blot analysis. (a) Expression level of indicated proteins was analyzed after incubating in normal culture medium, high-glucose culture medium, high-glucose culture medium with 1 μM or 10 μM Mcc950 supplementation. (b–d) Expression is shown relatively to β-actin in each group, which was set to 1. Data are shown as mean±S.E.M., n=3 technical replicates. Significant differences were calculated using one-way ANOVA followed by Tukey’s multiple comparison test. *P<0.05 versus control, ^#P<0.05 versus high-glucose group. HG, high glucose