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. 2017 Jul 20;8(7):e2941. doi: 10.1038/cddis.2017.308

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Immunofluorescence analysis of the expression of NLRP3 and IL-1β in HRECs. (a) Representative immunostaining images showed the NLRP3/ IL-1β double labeling HRECs induced by three different culture media (scale bar of 50 μm). (b) Quantitative analysis of the ratio of NLRP3/IL-1β co-labeling cell to total cell after incubating in three different culture media. Data are shown as mean±S.E.M., n=3 technical replicates. Significant differences were calculated using one-way ANOVA followed by Tukey’s multiple comparison test. *P<0.01 versus control, ^#P<0.05 versus high-glucose group