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. 2017 Jul 13;8(7):e2927. doi: 10.1038/cddis.2017.317

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Variation in autophagy flux between different leukemic cell lines. (a) Relative accumulation of autophagosomes after overnight treatment with 20 μM HCQ measured by staining with Cyto-ID in a panel of leukemic cell lines (N=7). (b) Representative FACS plots showing mean fluorescent intensity of Cyto-ID, with or without HCQ treatment. (c) mCherry/GFP ratio in a panel of leukemic cell lines transduced with mCherry-GFP-LC3. (d) Representative western blot of LC3-II accumulation after HCQ in cell lines, β-actin was used as loading control. Error bars represent S.D.; *, ** or *** represents P<0.05, P<0.01 or P<0.001, respectively