Figure 6.

Autophagy is higher in the ROS^low population of AML blasts. (a) Representative FACS plots showing CellROX staining in freshly isolated AML CD34⁺ cells. (b) Relative Cyto-ID levels in ROS^high and ROS^low fractions of AML CD34⁺ cells (n=11). (c) Gene expression of Beclin-1 and MAP1LC3A in freshly sorted AML CD34⁺ROS^high and CD34⁺ROS^low cells. (d) Survival of FACS-sorted ROS^high and ROS^low AML CD34⁺ cells, cultured for 3 days on an MS5 stromal layer before treated for 48 h with different concentrations HCQ. Error bars represent S.D., * or ** represents P<0.05 or P<0.01 respectively