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. 2017 Jul 13;8(7):e2919. doi: 10.1038/cddis.2017.318

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Autophagic turnover in the cortex is impaired after HI. (a) Western blot analysis of ubiquitin (UBQ) and SQSTM1/p62 in cortical tissue lysates from sham-operated and injured animals. (b, c) Densitometric analysis of (b) total ubiquitinated proteins and (c) SQSTM1/p62 with respect to the loading control, β-actin (n=4, **P<0.01). (d) Images of sensorimotor cortical sections stained with antibodies against LC3 and SQSTM1/p62. Scale bar=50 μm for low power images and 10 μm for high power insets. (e) Relative mRNA level of SQSTM1/p62 in the cortices of uninjured control and injured piglets (n=3). (f) Quantification of immunofluorescence data showing the number of SQSTM1/p62-positive cells in cortical brain sections from sham-operated and HI piglets (n=3, *P<0.05 at 1 d after HI). (g) Quantification of LC3-positive cells, and LC3 and SQSTM1/p62 double-positive cells. The percentages of double-positive versus single-positive cells are indicated (n=3, **P<0.01 at 1 d after HI; at least 1000 cells were quantified per piglet per experiment)