Figure 6.

Autophagy impairment contributes to cell death in cultured neurons. (a) Western blot analysis of LC3-I/II and p62 in primary cultured neurons treated with chloroquine (5 μmol/l) with or without rapamycin (100 nmol/l) treatment for 1 d. (b, c) Corresponding densitometric analysis of (b) LC3-I/II and (c) p62 bands with respect to β-actin (n=4, **P<0.01). (d) Cell death in primary cultured neonatal cortical neurons treated with chloroquine (5 μmol/l) with or without rapamycin (100 nmol/l) treatment and nutrient deprivation (ND) for 1 d (***P<0.001 versus the non-chloroquine treatment group). (e) Cell death in primary cultured neurons treated with chloroquine (5 μmol/l) with or without rapamycin (100 nmol/l) treatment for 1 d in the presence of Z-VAD-fmk (10 μmol/l), cyclosporine A (CsA; 10 μmol/l), or DMSO (control). *P<0.05, *P<0.01, versus control, #P<0.05 versus the chloroquine treatment group, $P<0.05, $$P<0.01 versus the rapamycin plus chloroquine treatment group