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. 2017 Jul 27;8(7):e2952. doi: 10.1038/cddis.2017.323

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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KLF5 inhibits ccRCC cell growth and migration/invasion in vitro. 786-O, A498 and RCC4 cells were stably infected with control (NC) or KLF5 lentivirus. (a) Western blots for KLF5 expression (left) and CCK8 analysis for proliferation (right) of indicated cells. (b) Representative colony formation of NC or KLF5-transduced 786-O, A498 and RCC4 cells (up) and statistical analyses of colony numbers (down). (c,d) Representative images of transwell migration (c) or invasion (d) assays of NC or KLF5-transduced 786-O, A498 and RCC4 cells (up) and statistical analyses of migrated or invaded cell numbers were shown (down). All experiments were repeated at least three times with triplicate samples. All bar graphs are plotted as mean±S.D. P-values are calculated between linked groups. *P<0.05; **P<0.01; ***P<0.001