Figure 1.

Upregulation of BAG3 expression in breast cancer correlated with poor patient survival. (a) BAG3 mRNA was evaluated in 137 pairs of breast cancer tissues compared with corresponding non-tumor breast specimens. BAG3 expression levels were calculated by the BAG3/18S rRNA expression ratio and plotted on the graph. (b) The BAG3 expression level between primary breast cancer and corresponding non-tumor breast specimens was compared. A log₂-fold change of more than +2 or less than −2 was considered as significant upregulation or downregulation (dotted lines). (c) Western blot analysis of BAG3 in protein extracts from breast cancer specimens. Data are representative immunoblots of three independent assays. (d) Representative immunohistochemistry staining with BAG3. (e) Kalpan–Meier plot indicates the overall survival of patients with breast cancer categorized by BAG3 expression; P-value is determined by log-rank test