Figure 7.

Positive correlation of BAG3 with CXCR4 in breast cancer. (a) CXCR4 mRNA was evaluated in 137 pairs of breast cancer tissues compared with corresponding non-tumor breast specimens. CXCR4 expression levels were calculated by the CXCR4/18S rRNA expression ratio and plotted on the graph. (b) The CXCR4 expression level between primary breast cancer and corresponding non-tumor breast specimens was compared. A log₂-fold change of more than +2 or less than −2 was considered as significant upregulation or downregulation (dotted lines). (c) Regression analysis was performed between the normalized BAG3 and CXCR4 mRNA. Each dot represents a sample, and the dotted line represents the linear regression fit, with the Pearson correlation coefficient (r) shown in the corner of the box. (d) Representative immunohistochemistry staining with BAG3 and CXCR4. (e) Kalpan–Meier plot indicates the overall survival of patients with breast cancer categorized by CXCR4 expression; P-value is determined by log-rank test. (f) Kalpan–Meier plot indicates the overall survival of patients with breast cancer categorized by combined BAG3 and CXCR4 expression; P-value is determined by log-rank test