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MiRNA expression changes in J1 ESCs induced by RA. (a) J1 ESCs were treated with RA for 48 h, and qRT-PCR was performed to determine the relative expression levels of 23 miRNAs. U6 served as the internal control. (b–d) Relative expression levels of miR-10-5p (b), miR-219 (c), and miR-219-2-3p (d) at different time points after RA treatment on ESCs. All data are presented as mean±S.D. and derived from three independent experiments
