Figure 2.

MiR-219 mediates ESCs to differentiate into neural cells. (a) Confirmation of the effects of miR-219 mimics and inhibitors. (b, c) ESCs were transfected with miR-219 mimics for 48 h; the relative levels of Oct4, Nestin, and Map2 were detected through qRT-PCR (b) and western blot (c). (d) Immunofluorescence shows the abundance of Oct4, Nestin, and Map2, as well as the morphologies of the ESCs after transfection with miR-219 mimics for 48 h. (e, f) ESCs were pretreated with RA for 48 h and transfected with miR-219 inhibitors. The relative levels of Oct4, Nestin, and Map2 were detected using qRT-PCR (e) and western blot (f). (g) Immunofluorescence shows the abundance of Oct4, Nestin, and Map2, as well as the morphologies of the ESCs pretreated with RA for 48 h and transfected with miR-219 inhibitors. **P<0.01; NS, no significance