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. 2017 Jul 27;8(7):e2953. doi: 10.1038/cddis.2017.336

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Olig1 and Olig2 are critical to miR-219-mediated neural differentiation. (a, b) Relative levels of Nestin, Map2, and Tuj1 were detected through qRT-PCR (a) and western blot (b) in ESCs transfected with Olig1/Olig2-encoding plasmids for 48 h. (c, d) ESCs were transfected with siRNAs that were targeted to Olig1/Olig2 for 12 h and treated with RA or miR-219 mimics for another 36 h. Relative levels of Nestin, Map2, and Tuj1 were detected through qRT-PCR (c) and western blot. (e) Immunofluorescence shows the abundance of Oct4, Nestin, and Map2, as well as the morphologies of ESCs transfected with Olig1/Olig2 or miR-291 mimics. **P<0.01; NS, no significance