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. 2017 Jul 27;8(7):e2961. doi: 10.1038/cddis.2017.339

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Knockdown of KRT23 inhibited the proliferative capacity and migration of LoVo CRC cells. (a) Cell viability was analyzed by MTT in LoVo cells that were transfected with siKRT23 or NSP for 48 h. (b) Colony formation assay of LoVo cells transfected with siKRT23 or NSP two times a week for 2 weeks. (c) Cell migration was analyzed by a wound-healing assay. LoVo cells were seeded in 6-well plates and grown to full confluence (50 ×). (d) The phosphorylated and total ERK and p38 levels in LoVo cells transfected with siKRT23 were detected by western blot assay using antibodies against p-p38, p38, p-ERK and ERK (*P<0.05, **P<0.01 and ***P<0.001)