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. 2017 Jul 27;8(7):e2961. doi: 10.1038/cddis.2017.339

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Overexpression of KRT23 promoted proliferation and migration in RKO CRC cells. (a) Cell viability was analyzed by MTT in RKO cells that were infected with or the NSP LV-NC. (b) Colony formation assay of RKO cells infected with LV-KRT23 or the NSP LV-NC. (c) Cell migration was analyzed by a wound-healing assay. RKO cells were seeded in 6-well plates and grown to full confluence (50 ×). (d) The phosphorylated and total ERK and p38 levels in RKO cells infected with LV-KRT23 were measured by a western blot assay using antibodies against p-p38, p38, p-ERK and ERK. (e) Cell viability was analyzed by CCK-8 in RKO cells that were treated with U0126 and/or LV-KRT23. (f) Cell viability was analyzed by CCK-8 in RKO cells that were treated with SB203580 and/or LV-KRT23. (g) Cell migration was analyzed by a wound-healing assay. RKO cells were treated with SB203580 and/or LV-KRT23. (h) Cell migration was analyzed by a wound-healing assay. RKO cells were treated with U0126 and/or LV-KRT23. (i) The phosphorylated and total p38 levels in RKO cells treated with SB203580 and/or LV-KRT23 were measured by a western blot assay using antibodies against p-p38 and p38. (j) The phosphorylated and total ERK 1/2 levels in RKO cells treated with U0126 and/or LV-KRT23 were measured by a western blot assay using antibodies against p-ERK 1/2 and ERK 1/2 (*P<0.05, **P<0.01 and ***P<0.001)