Figure 3.

The differential response of WWOX-transfected cells to paclitaxel is not related to the antimitotic action of taxanes, and WWOX-induced apoptosis is independent of ITGA3 and TGFBI. (a) WWOX transfection does not alter the percentage of mitotic (MPM2 antibody-labelled (Millipore)) cells as determined by FACS following treatment with paclitaxel. Means of triplicate experiments±S.E.M. (b) WWOX depletion does not alter the cytotoxic response to monastrol. Cell survival following treatment with monastrol for 72 h, measured by SRB assay. Means of triplicate experiments±S.E.M. (c) ITGA3 expression following siRNA knockdown as measured by FACS, compared with mock-transfected and non-targeting siRNA. Means of quadruplicate experiments±S.E.M. (d) ITGA3 siRNA knockdown did not affect cell survival following exposure with 8 nM paclitaxel, measured by SRB assay. Means of six experiments±S.E.M. (e) TGFBI mRNA expression is not related to WWOX expression in WWOX- and vector-transfected PEO1 lines. Means of six experiments±S.E.M. NT, non-targeting siRNA