Figure 4.

Paclitaxel evokes ER stress in PEO1 cells and WWOX sensitises PEO1 cells to the ER stress inducer tunicamycin. (a) WWOX-8 and control Vector-9 cells were treated with 8 nM paclitaxel or (b) 200 ng/μl tunicamycin. Floating and adherent cells were lysed at 0, 1, 2, 4, 8, 12, 18, 24 and 32 h time points and analysed by western blotting. (c) Relative cell survival following treatment with tunicamycin for 48 h, measured by SRB assay. Means of triplicate experiments±S.E.M. (d and e) Knockdown of WWOX in WWOX-8 cells decreases apoptosis following treatment with tunicamycin, compared with mock-transfected or non-targeting controls. Relative cell survival following exposure with tunicamycin for 48 h, measured by SRB assay. Means of quadruplicate experiments±S.E.M. (*P<0.05; **P<0.01; ***P<0.005). NT, non-targeting siRNA